Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies

4.2K Views

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14:56 min

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May 6th, 2022

DOI :

10.3791/62598-v

May 6th, 2022

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Natália Yumi Noronha*¹, Guilherme da Silva Rodrigues*¹, Marcela Augusta de Souza Pinhel¹, Jean-Baptiste Cazier²^,³, Lígia Moriguchi Watanabe¹, Albert Nobre Menezes⁴, Carlos Roberto Bueno⁵, Carolina Ferreira Nicoletti¹, Bruno Affonso Parenti de Oliveira¹, Isabelle Mello Schineider⁶, Isabella Harumi Yonehara Noma⁷, Igor Caetano Dias Alcarás⁸, Fernando Barbosa⁹, Carla Barbosa Nonino⁶

¹Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, ²Centre for Computational Biology, University of Birmingham, ³Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham, ⁴Cancer Genetics and Evolution Laboratory, Cancer Research UK, Institute of Genetics & Molecular Medicine, The University of Edinburgh, ⁵Ribeirão Preto School of Physical Education and Sport, University of São Paulo, ⁶Health Sciences Department, Ribeirao Preto Medical School, University of Sao Paulo, ⁷Faculty of Pharmaceutical Sciences, University of São Paulo, ⁸Department of Genetics, Ribeirão Preto Medical School, University of São Paulo, ⁹Department of Clinical, Bromatological and Toxicological Analysis, Faculty of Pharmaceutical Sciences of Ribeirao Preto, University of São Paulo

Summary

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DNA Methylation

Bioinformatics Analysis

Methylation Assay Protocol

Chapters in this video

0:03

Introduction

0:43

Day 1: Bisulfite Treatment

1:21

Day 2: Amplification - Methylation Assay, Manual Protocol

2:52

Day 3: Hybridization - Methylation Assay, Manual Protocol

6:39

Day 4: Staining - Methylation Assay, Manual Protocol

10:48

Representative Results

14:34

Conclusion

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