An Automated Culture System for Use in Preclinical Testing of Host-Directed Therapies for Tuberculosis
1.8K Views
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09:34 min
•
August 16th, 2021
DOI :
August 16th, 2021
•Seónadh O’Leary1, Ahmad Z. Bahlool1,2, Gemma O’Connor2, Sally-Ann Cryan2,3,4, Joseph M. Keane1, Mary P. O’Sullivan1
1Department of Clinical Medicine, Trinity Translational Medicine Institute, St. James's Hospital, Trinity College Dublin, The University of Dublin, 2School of Pharmacy and Biomolecular Sciences, Royal College of Surgeons in Ireland (RCSI), 3SFI Advanced Materials and Bioengineering Research (AMBER) Centre, RCSI & TCD, 4SFI Centre for Research in Medical Devices (CURAM), RCSI, Dublin and National University of Ireland
Chapters in this video
0:04
Introduction
0:42
Determination of Mycobacterium tuberculosis (Mtb) Uptake by Macrophages: Multiplicity of Infection (MOI)
2:30
Acid Fast Bacteria Staining
4:14
Macrophage Infection
5:09
Sample Collection
6:10
Inoculating and Loading Instrument Culture Bottles
7:22
Results:Mycobacterium tuberculosis Growth Quantification and Therapy Evaluation Using Automated Culture System
8:54
Conclusion
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