A Standardized Procedure for Monitoring Harmful Algal Blooms in Chile by Metabarcoding Analysis

5.0K Views

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09:47 min

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August 26th, 2021

DOI :

10.3791/62967-v

August 26th, 2021

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Kyoko Yarimizu¹, So Fujiyoshi¹, Mikihiko Kawai², Jacquelinne J. Acuña³, Joaquin-Ignacio Rilling³, Marco Campos³, Jonnathan Vilugrón⁴, Henry Cameron⁵, Karen Vergara⁶, Gonzalo Gajardo⁶, Oscar Espinoza-González⁴, Leonardo Guzmán⁴, Satoshi Nagai⁷, Carlos Riquelme⁵, Milko A. Jorquera³, Fumito Maruyama¹

¹Office of Research and Academia-Government-Community Collaboration, Hiroshima University, ²Graduate School of Human and Environmental Studies, Kyoto University, ³Scientific and Biotechnological Bioresource Nucleus, Universidad de La Frontera, ⁴Centro de Estudios de Algas Nocivas, Instituto de Fomento Pesquero, ⁵Ciencias del Mar y Recursos Biologicos, Universidad de Antofagasta, ⁶Departamento de Ciencias Biológicas y Biodiversidad, Universidad de Los Lagos, ⁷Japan Fisheries Research and Education Agency, Fisheries Resources Institute

Summary

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Harmful Algal Blooms

Metabarcoding Analysis

Toxic Algal Species

Water Sample Collection

Phytoplankton Species

DNA Extraction

16S RRNA Analysis

18S RRNA Analysis

PCR Amplicon Generation

Electrophoresis

Laminar Hood Cabinet

DNA Cleanup System

Chapters in this video

0:04

Introduction

0:59

Sample Collection, Pretreatment, and Microscope Analysis

1:55

16S rRNA and 18S rRNA Metabarcoding Analysis

7:00

Results: Metabarcoding Analysis for Monitoring Harmful Algal Blooms and Their Associated Microbiome in Seawater

8:50

Conclusion

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