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Generating Transgenics and Knockouts in Strongyloides Species by Microinjection Video (Video) | JoVE

DOI :

10.3791/63023-v

October 7th, 2021

October 7th, 2021

2,272 Views

1Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, 2Molecular Biology Institute, University of California, Los Angeles

The functional genomic toolkit for the parasitic nematodes Strongyloides stercoralis and Strongyloides ratti includes transgenesis, CRISPR/Cas9-mediated mutagenesis, and RNAi. This protocol will demonstrate how to use intragonadal microinjection to introduce transgenes and CRISPR components into S. stercoralis and S. ratti.

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