October 7th, 2021
•Cryo-section-dissection allows fresh, frozen preparation of the largest neurogenic niche in the murine brain for deep quantitative proteome analysis. The method is precise, efficient, and causes minimal tissue perturbation. Therefore, it is ideally suited for studying the molecular microenvironment of this niche, as well as other organs, regions, and species.
Related Videos
The Subventricular Zone En-face: Wholemount Staining and Ependymal Flow
Cerebrovascular Casting of the Adult Mouse for 3D Imaging and Morphological Analysis
Enabling High Grayscale Resolution Displays and Accurate Response Time Measurements on Conventional Computers
Dissection of Adult Mouse Utricle and Adenovirus-mediated Supporting-cell Infection
Dissection and Immunohistochemistry of Larval, Pupal and Adult Drosophila Retinas
Methods for the Modulation and Analysis of NF-κB-dependent Adult Neurogenesis
Dissection of the Transversus Abdominis Muscle for Whole-mount Neuromuscular Junction Analysis
Cell Sorting of Neural Stem and Progenitor Cells from the Adult Mouse Subventricular Zone and Live-imaging of their Cell Cycle Dynamics
Whole Mount Dissection and Immunofluorescence of the Adult Mouse Cochlea
Isolation and Culture of Adult Neural Stem Cells from the Mouse Subcallosal Zone
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved