JoVE Logo
Faculty Resource Center

Sign In

Cryo-section Dissection of the Adult Subependymal Zone for Accurate and Deep Quantitative Proteome Analysis

DOI :

10.3791/63047-v

6:24 min

October 7th, 2021

October 7th, 2021

3,023 Views

1Division of Physiological Genomics, Biomedical Center, Ludwig Maximilian University of Munich, 2Institute for Stem Cell Research, Helmholtz Zentrum München, 3SYNERGY, Excellence Cluster Systems Neurology, University of Munich, 4Department of Clinical Neuroscience, Karolinska Institutet

Cryo-section-dissection allows fresh, frozen preparation of the largest neurogenic niche in the murine brain for deep quantitative proteome analysis. The method is precise, efficient, and causes minimal tissue perturbation. Therefore, it is ideally suited for studying the molecular microenvironment of this niche, as well as other organs, regions, and species.

Tags

Cryo section Dissection

-- Views

Related Videos

article

The Subventricular Zone En-face: Wholemount Staining and Ependymal Flow

article

Cerebrovascular Casting of the Adult Mouse for 3D Imaging and Morphological Analysis

article

Enabling High Grayscale Resolution Displays and Accurate Response Time Measurements on Conventional Computers

article

Dissection of Adult Mouse Utricle and Adenovirus-mediated Supporting-cell Infection

article

Dissection and Immunohistochemistry of Larval, Pupal and Adult Drosophila Retinas

article

Methods for the Modulation and Analysis of NF-κB-dependent Adult Neurogenesis

article

Dissection of the Transversus Abdominis Muscle for Whole-mount Neuromuscular Junction Analysis

article

Cell Sorting of Neural Stem and Progenitor Cells from the Adult Mouse Subventricular Zone and Live-imaging of their Cell Cycle Dynamics

article

Whole Mount Dissection and Immunofluorescence of the Adult Mouse Cochlea

article

Isolation and Culture of Adult Neural Stem Cells from the Mouse Subcallosal Zone

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved