Shotgun Proteomics Sample Processing Automated by an Open-Source Lab Robot

3.5K Views

10:12 min

October 28th, 2021

DOI :

10.3791/63092-v

October 28th, 2021


Transcript

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Shotgun Proteomics

Chapters in this video

0:04

Introduction

0:40

Mass Spectrometry (MS) Sample Preparation with a Single Protein

4:44

Peptide Clean-up Using SP3 Paramagnetic Beads

6:19

MS Sample Preparation with Protein Lysate

8:37

Results: Mass Spectrometry Proteomics with a Single Protein Standard Bovine Serum Albumin and a Detergent-Containing Human Heart Lysate Sample

9:44

Conclusion

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