Integrative Toolkit to Analyze Cellular Signals: Forces, Motion, Morphology, and Fluorescence

3.8K Views

•

14:55 min

•

March 5th, 2022

DOI :

10.3791/63095-v

March 5th, 2022

•

Alyson Nguyen*¹, Keith Battle*²^,³, Sunita S. Paudel*²^,³, Ningyong Xu², Jessica Bell³, Linn Ayers³, Cassandra Chapman⁴, Ajay P. Singh⁵, Srinivas Palanki⁶, Thomas Rich³^,⁷, Diego F. Alvarez⁸, Troy Stevens²^,³, Dhananjay T. Tambe³^,⁴^,⁷

¹Biomedical Sciences, Pat Capps Covey College of Allied Health Professions, University of South Alabama, ²Department of Physiology and Cell Biology, College of Medicine, University of South Alabama, ³Center for Lung Biology, College of Medicine, University of South Alabama, ⁴William B. Burnsed Jr. Mechanical, Aerospace, and Biomedical Engineering Department, College of Engineering, University of South Alabama, ⁵Mitchell Cancer Institute, College of Medicine, University of South Alabama, ⁶Department of Chemical and Biomedical Engineering, West Virginia University, ⁷Department of Pharmacology, College of Medicine, University of South Alabama, ⁸Department of Physiology & Pharmacology, College of Osteopathic Medicine, Sam Houston State University

Summary

Explore More Videos

Monolayer Stress Microscopy

Confluence Cell Monolayer

Phase Contrast Imaging

Data Visualization

Chapters in this video

0:02

Introduction

1:46

Creation of Position List

3:57

Automated Image Acquisition for the Remaining Experiment

4:34

Setting Up Automated Data Analysis

6:08

Quantification of Hydrogel Deformation

6:37

Quantification of Cell-ECM and Cell-cell Forces

8:44

Segmentation of the Individual Cells in the Images

9:47

Mapping of the Pixel Intensities on the Cells

11:06

Mapping of the Forces and Motion on the Cells

11:51

Tracking of the Cellular Indentities across the Experiment

12:51

Generation of Time Tracks of the Assessed Cellular Properties

13:27

Generation of the Heat Maps of the Assessed Cellular Properties

13:53

Representative Results

14:30

Conclusion

Related Videos

12:30

Testing Visual Sensitivity to the Speed and Direction of Motion in Lizards

11.4K Views

07:18

Microinjection of Zebrafish Embryos to Analyze Gene Function

77.1K Views

07:29

A high-throughput method to globally study the organelle morphology in S. cerevisiae

10.3K Views

08:21

Time-lapse Imaging of Mitosis After siRNA Transfection

16.9K Views

10:00

An Analytical Tool that Quantifies Cellular Morphology Changes from Three-dimensional Fluorescence Images

14.5K Views

10:00

Measurement of Lifespan in Drosophila melanogaster

33.9K Views

07:00

Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae

34.6K Views

08:46

Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium

9.7K Views

10:33

Local Field Fluorescence Microscopy: Imaging Cellular Signals in Intact Hearts

8.2K Views

08:12

A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions

7.3K Views