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JoVE Journal

Developmental Biology

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Analysis of Cell Differentiation, Morphogenesis, and Patterning During Chicken Embryogenesis Using the Soaked-Bead Assay

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06:49 min

January 12th, 2022

January 12th, 2022

2,954 Views

0:04

Introduction

0:48

Egg Incubation and Embryo Staging

2:08

Bead Preparation

4:34

Embryo Manipulation and Interdigit Implantation

5:42

Results: Evaluation of Cell Behavior in the Embryonic Chick Limb Using Soaked Beads

6:20

Conclusion

Transcript

This assay enables the student to look specific molecules in many developmental process, such as cell differentiation and morphogenesis by implanting the beads into distinct embryo regions. This protocol can be applied to any experimental animal m

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The soaked bead assay involves targeted delivery of test reagent at any developmental time point to study the regulation of cell differentiation and morphogenesis. A detailed protocol, applicable to any experimental animal model, for preparing three different types of soaked beads and implanting these in the interdigit of a chicken embryo is presented.

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