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Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

3.4K Views

06:43 min

May 3rd, 2022

DOI :

10.3791/63730-v

May 3rd, 2022


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Simultaneous Interference Reflection

Chapters in this video

0:05

Introduction

0:29

Preparation of PDMS Channels

2:15

Preparation for Imaging

3:02

Growing Dynamic Guanosine Diphosphate “Extensions”

3:46

Kinesin Motility Assay

4:23

Image Processing and Analysis

5:22

Results: Imaging of Dynamic Microtubules and Fluorescently Labeled Microtubule-Associated Proteins

5:57

Conclusion

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