Results: Quantification of Senescence-associated Markers
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Conclusion
Transcript
This method can help answer key questions in the aging and cancer field such as how to perform common techniques to induce cellular senescence and how to evaluate the senescence phenotype. The main advantages of this technique are that it is easy
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Here, we discuss a series of protocols for induction and validation of cellular senescence in cultured cells. We focus on different senescence-inducing stimuli and describe the quantification of common senescence-associated markers. We provide technical details using fibroblasts as a model, but the protocols can be adapted to various cellular models.