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Induction and Validation of Cellular Senescence in Primary Human Cells

DOI :

10.3791/57782-v

June 20th, 2018

June 20th, 2018

15,818 Views

1European Research Institute for the Biology of Aging, University of Groningen, University Medical Center Groningen

Here, we discuss a series of protocols for induction and validation of cellular senescence in cultured cells. We focus on different senescence-inducing stimuli and describe the quantification of common senescence-associated markers. We provide technical details using fibroblasts as a model, but the protocols can be adapted to various cellular models.

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Keywords Cellular Senescence

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