JoVE Logo
Faculty Resource Center

Sign In

Generation of a Simplified Three-Dimensional Skin-on-a-chip Model in a Micromachined Microfluidic Platform

DOI :

10.3791/62353-v

May 17th, 2021

May 17th, 2021

3,122 Views

1Department of Bioengineering and Aerospace Engineering, Universidad Carlos III de Madrid (UC3M), 2Group of Optics, Photonics and Biophotonics (GOFB). Center for Biomedical Technology, Universidad Politécnica de Madrid, 3Division of Epithelial Biomedicine, CIEMAT, 4Instituto de Investigación Sanitaria Gregorio Marañón

Here, we present a protocol to generate a three-dimensional simplified and undifferentiated skin model using a micromachined microfluidic platform. A parallel flow approach allows the in situ deposition of a dermal compartment for the seeding of epithelial cells on top, all controlled by syringe pumps.

-- Views

Related Videos

article

Alginate Microcapsule as a 3D Platform for Propagation and Differentiation of Human Embryonic Stem Cells (hESC) to Different Lineages

article

Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies

article

Quantitative Characterization of Liquid Photosensitive Bioink Properties for Continuous Digital Light Processing Based Printing

article

A Novel Cell Injection Method with Minimum Invasion

article

Soybean Hairy Root Transformation for the Analysis of Gene Function

article

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

article

Author Spotlight: Advances in Evaluating Human Lung Epithelial Cells' Response to Metal-Organic Frameworks

article

Simple Establishment of a Vascularized Osteogenic Bone Marrow Niche Using Pre-Cast Poly(ethylene Glycol) (PEG) Hydrogels in an Imaging Microplate

article

Methods for Electroporation and Transformation Confirmation in Limosilactobacillus reuteri DSM20016

article

Author Spotlight: Advancing Eye Physiology Research via a Multi-Channel Flow Culture for Optimal Tissue Maintenance and Real-Time Assessment

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved