Whole-Brain Single-Cell Imaging and Analysis of Intact Neonatal Mouse Brains Using MRI, Tissue Clearing, and Light-Sheet Microscopy

3.4K Views

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08:49 min

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August 1st, 2022

DOI :

10.3791/64096-v

August 1st, 2022

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Felix A. Kyere*¹^,², Ian Curtin*¹^,², Oleh Krupa¹^,², Carolyn M. McCormick¹^,², Mustafa Dere³, Sarah Khan³^,⁷, Minjeong Kim⁷, Tzu-Wen Winnie Wang⁴^,⁵^,⁶, Qiuhong He⁴^,⁵^,⁶, Guorong Wu³, Yen-Yu Ian Shih⁴^,⁵^,⁶, Jason L. Stein¹^,²

¹UNC Neuroscience Center, University of North Carolina, Chapel Hill, ²Department of Genetics, University of North Carolina, Chapel Hill, ³Department of Psychiatry, University of North Carolina, Chapel Hill, ⁴Center for Animal Magnetic Resonance Imaging, The University of North Carolina at Chapel Hill, ⁵Biomedical Research Imaging Center, The University of North Carolina at Chapel Hill, ⁶Department of Neurology, The University of North Carolina at Chapel Hill, ⁷Department of Computer Science, The University of North Carolina at Greensboro

Summary

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Light sheet Microscopy

Image Analysis Tools

Computational Pipeline

Cell Detection Accuracy

UltraMicroscope II

Horizontal Dynamic Focusing

Laser Wavelength

Image Processing Tools

Conda Environment Manager

NuMorph Software

Imaging Parameters

Chapters in this video

0:05

Introduction

0:48

Light-Sheet Imaging

2:25

Image Processing Using NuMorph

4:12

Image Analysis

6:26

Results: Quantification of Nuclear Markers Within Mouse Brain After Imaging on a Light-Sheet Microscope Using NuMorph

7:57

Conclusion

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