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Culture and Imaging of Ex Vivo Organotypic Pseudomyxoma Peritonei Tumor Slices from Resected Human Tumor Specimens

1.6K Views

09:19 min

December 9th, 2022

DOI :

10.3791/64620-v

December 9th, 2022


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Ex Vivo Organotypic

Chapters in this video

0:04

Introduction

0:48

Preparation of Human Pseudomyxoma Peritonei (PMP) Tissues for Tissue Processing and Culture

3:07

Confocal Imaging of Living Human Tissue Slices

3:51

Disassociation of Living Slices for Flow Cytometry

5:23

Pharmacological Intervention Using Living Tissue Slices for Viability and Proliferation Analysi

6:31

Results: Real-Time Visualization and Interrogation of Tumor Using Patient-Derived Ex Vivo Organotypic Slices

8:43

Conclusion

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