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JoVE Journal

Cancer Research

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Culture and Imaging of Ex Vivo Organotypic Pseudomyxoma Peritonei Tumor Slices from Resected Human Tumor Specimens

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09:19 min

December 9th, 2022

December 9th, 2022

1,527 Views

0:04

Introduction

0:48

Preparation of Human Pseudomyxoma Peritonei (PMP) Tissues for Tissue Processing and Culture

3:07

Confocal Imaging of Living Human Tissue Slices

3:51

Disassociation of Living Slices for Flow Cytometry

5:23

Pharmacological Intervention Using Living Tissue Slices for Viability and Proliferation Analysi

6:31

Results: Real-Time Visualization and Interrogation of Tumor Using Patient-Derived Ex Vivo Organotypic Slices

8:43

Conclusion

필기록

This research demonstrates a novel approach to the study of appendiceal cancer therapeutics and pathobiology in a preclinical setting. These methods may be more broadly applicable to the study of other malignancies. Appendiceal cancer is difficult

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We describe a protocol for the production, culture, and visualization of human cancers, which have metastasized to the peritoneal surfaces. Resected tumor specimens are cut using a vibratome and cultured on permeable inserts for increased oxygenation and viability, followed by imaging and downstream analyses using confocal microscopy and flow cytometry.

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