Results: Characterization of Emulsions Produced and EAE Induction in Immunized Mice
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Conclusion
Transcript
Experimental autoimmune encephalomyelitis or EAE is an animal model used to develop new and improved treatments for multiple sclerosis patient. Inducing disease in EAE requires a perfect oil and water emulsion to achieve consistent results. The me
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To induce experimental autoimmune encephalomyelitis, an animal model of multiple sclerosis, mice are immunized with a water-in-oil emulsion containing an autoantigen and complete Freund's adjuvant. While several protocols exist for the preparation of these emulsions, a rapid, simple, and standardized homogenization protocol for emulsion preparation is presented here.