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An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity

DOI :

10.3791/58100-v

October 8th, 2018

October 8th, 2018

6,696 Views

1Department of Biology, Ball State University, 2Department of Genome Sciences, University of Washington, 3Department of Molecular, Cellular, and Developmental Biology, University of Michigan

Here, we describe a protocol for the biochemical characterization of the yeast RNA-modifying enzyme, Mod5, and discuss how this protocol could be applied to other RNA-modifying enzymes.

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