Isolation and Culture of Human Adipose-Derived Stem Cells With an Innovative Xenogeneic-Free Method for Human Therapy
3.0K Views
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06:00 min
•
February 3rd, 2023
DOI :
February 3rd, 2023
•Martino Guiotto*1,4, Silvia Palombella*2, Stefania Brambilla2, Lee Ann Applegate3, Mathis Riehle4, Andrew Hart4, Wassim Raffoul1, Pietro Giovanni di Summa1
1Department of Plastic, Reconstructive and Hand Surgery, Centre Hospitalier Universitaire Vaudois (CHUV, 2Cell and Tissue Engineering Lab, IRCCS Ospedale Galeazzi Sant'Ambrogio, 3Unit of Regenerative Therapy, Plastic, Reconstructive and Hand Surgery, Department of Musculoskeletal Medicine, Lausanne University Hospital, 4Centre for the Cellular Microenvironment, University of Glasgow
Chapters in this video
0:04
Introduction
1:04
Human Adipose-Derived Stem Cells (hASCs) Isolation from Adipose Tissue
2:21
hASC Culture, Cryopreservation, and Storage
4:25
Results: Assessment of Human Platelet Lysate (hPL)-Induced hASCs
5:22
Conclusion
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