Disruption of the Blood-Spinal Cord Barrier Using Low-Intensity Focused Ultrasound in a Rat Model
1.9K Views
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09:03 min
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March 10th, 2023
DOI :
March 10th, 2023
•Meghana Bhimreddy1, Denis Routkevitch1,2,3, Andrew M. Hersh1, Ali Mohammadabadi1,3, Arjun K. Menta1, Kelly Jiang1, Carly Weber-Levine1, A. Daniel Davidar1, Joshua Punnoose1,2,3, Kelley M. Kempski Leadingham1,3, Joshua C. Doloff2, Betty Tyler1, Nicholas Theodore1,3, Amir Manbachi1,2,3,4,5,6
1Department of Neurosurgery, Johns Hopkins University School of Medicine, 2Department of Biomedical Engineering, Johns Hopkins University, 3HEPIUS Innovation Laboratory, Johns Hopkins University School of Medicine, 4Department of Electrical Engineering and Computer Science, Johns Hopkins University, 5Department of Mechanical Engineering, Johns Hopkins University, 6Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University
Chapters in this video
0:04
Introduction
1:11
Low‐Intensity Focused Ultrasound Assembly and Setup
2:20
Animal Preparation and Surgical Laminectomy
3:38
Target Localization Using Laser Guidance
4:22
Microbubble Administration
5:27
Spinal Cord Extraction, Tissue Processing, and Visualization of BSCB Disruption
6:34
Results: Confirmation of Target Localization and Evaluating BSCB Disruption by EBD Extravasation into the Spinal Cord
8:17
Conclusion
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