Disruption of the Blood-Spinal Cord Barrier Using Low-Intensity Focused Ultrasound in a Rat Model

DOI :

10.3791/65113-v

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9:03 min

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March 10th, 2023

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1,348 Views

Meghana Bhimreddy¹, Denis Routkevitch^1,2,3, Andrew M. Hersh¹, Ali Mohammadabadi^1,3, Arjun K. Menta¹, Kelly Jiang¹, Carly Weber-Levine¹, A. Daniel Davidar¹, Joshua Punnoose^1,2,3, Kelley M. Kempski Leadingham^1,3, Joshua C. Doloff², Betty Tyler¹, Nicholas Theodore^1,3, Amir Manbachi^1,2,3,4,5,6

¹Department of Neurosurgery, Johns Hopkins University School of Medicine, ²Department of Biomedical Engineering, Johns Hopkins University, ³HEPIUS Innovation Laboratory, Johns Hopkins University School of Medicine, ⁴Department of Electrical Engineering and Computer Science, Johns Hopkins University, ⁵Department of Mechanical Engineering, Johns Hopkins University, ⁶Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University

Chapters

Disruption of the blood-spinal cord barrier (BSCB) can be successfully achieved with the intravenous administration of microbubbles and the application of low-intensity focused ultrasound (LIFU). This protocol details the opening of the BSCB using LIFU in a rodent model, including equipment setup, microbubble injection, target localization, and BSCB disruption visualization.

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