Daily Transfers, Archiving Populations, and Measuring Fitness in the Long-Term Evolution Experiment with Escherichia coli
3.1K Views
•
15:00 min
•
August 18th, 2023
DOI :
August 18th, 2023
•Jeffrey E. Barrick1,2, Zachary D. Blount2,3, Devin M. Lake2,4, Jack H. Dwenger1, Jesus E. Chavarria-Palma1, Minako Izutsu2,3, Michael J. Wiser2,5
1Department of Molecular Biosciences, The University of Texas at Austin, 2BEACON Center for the Study of Evolution in Action, Michigan State University, 3Department of Microbiology and Molecular Genetics, Michigan State University, 4Department of Integrative Biology, Michigan State University, 5Biological Sciences Program, Michigan State University
Chapters in this video
0:03
Introduction
2:02
Daily Transfers of LTEE Populations
6:04
Competitive Fitness Assays
6:44
Prepare Supplies
8:24
Day -2: Revive Competitors
9:09
Day -1: Precondition Competitors
9:47
Day 0: Begin Competition
11:16
Alternative:Continue Three-Day Competition
11:43
Day 1 or 3: Finish Competition
12:41
Calculate and Plot Fitness
12:52
Representative Results Appearance and turbidity of LTEE Cultures
13:22
Representative Results Co-culture Competition Results
14:39
Conclusion
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