JoVE Logo
Faculty Resource Center

Sign In

Daily Transfers, Archiving Populations, and Measuring Fitness in the Long-Term Evolution Experiment with Escherichia coli

DOI :

10.3791/65342-v

15:00 min

August 18th, 2023

August 18th, 2023

1,919 Views

1Department of Molecular Biosciences, The University of Texas at Austin, 2BEACON Center for the Study of Evolution in Action, Michigan State University, 3Department of Microbiology and Molecular Genetics, Michigan State University, 4Department of Integrative Biology, Michigan State University, 5Biological Sciences Program, Michigan State University

This protocol describes how to maintain the Escherichia coli Long-Term Evolution Experiment (LTEE) by performing its daily transfers and periodic freeze-downs and how to conduct competition assays to measure fitness improvements in evolved bacteria. These procedures can serve as a template for researchers starting their own microbial evolution experiments.

Tags

Daily Transfers

-- Views

Related Videos

article

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

article

Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance

article

Producing Gene Deletions in Escherichia coli by P1 Transduction with Excisable Antibiotic Resistance Cassettes

article

A FACS-based Protocol to Isolate RNA from the Secondary Cells of Drosophila Male Accessory Glands

article

Measuring Microbial Mutation Rates with the Fluctuation Assay

article

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

article

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

article

An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

article

Author Spotlight: RNAi Inheritance and ChIP in C. elegans

article

Exercise Test for Evaluation of the Functional Efficacy of the Pig Cardiovascular System

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved