Rapid In Vitro Cytotoxicity Evaluation of Jurkat Expressing Chimeric Antigen Receptor using Fluorescent Imaging
2.2K Views
•
09:20 min
•
October 27th, 2023
DOI :
October 27th, 2023
•Siddharth Subham*1,2,3, John D. Jeppson*1, Benjamin K. Gibbs4, Jacqueline Babai2, Riza Alker1, Andrew K. Godwin4,5,6, David Akhavan1,2,3
1Department of Radiation Oncology, University of Kansas Cancer Center, 2Department of Cancer Biology, University of Kansas Cancer Center, 3BioEngineering Program, University of Kansas, 4Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, 5University of Kansas Cancer Center, 6Kansas Institute for Precision Medicine, University of Kansas Medical Center
Chapters in this video
0:00
Introduction
1:42
Plating CFSE‐Labelled Tumor Cells and Co‐Culturing CAR‐Expressing Jurkats for In Vitro Cytotoxicity Evaluation
5:19
Preparation of Plate for Fluorescent Imaging and Cell Viability Assessment in CAR‐Jurkat Co‐Culture with CFSE‐Labeled Tumor Cells
Related Videos
09:34
Dynamic Imaging of Chimeric Antigen Receptor T Cells with [18F]Tetrafluoroborate Positron Emission Tomography/Computed Tomography
3.3K Views
13:19
Quantifying Antibody-Dependent Cellular Cytotoxicity in a Tumor Spheroid Model: Application for Drug Discovery
2.5K Views
07:37
Performing an In Vitro Genome-Wide CRISPR Knockout Screen in Chimeric Antigen Receptor T Cells
778 Views
07:52
Detection of Antibodies That Neutralize the Cellular Uptake of Enzyme Replacement Therapies with a Cell-based Assay
8.8K Views
08:46
A Real-time Potency Assay for Chimeric Antigen Receptor T Cells Targeting Solid and Hematological Cancer Cells
53.1K Views
09:37
Imaging the Human Immunological Synapse
14.1K Views
08:09
Droplet-based Cytotoxicity Assay to Assess Chimeric Antigen Receptor T cells at the Single-cell Level
735 Views
06:56
A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4
13.6K Views
01:36
An In Vitro Technique for UV Irradiation-Mediated Generation of Apoptotic T Cells
537 Views
01:25
An In Vitro Assay to Assess CAR T Cell Cytotoxicity against Tumor Spheroids
95 Views
Copyright © 2025 MyJoVE Corporation. All rights reserved