Rapid In Vitro Cytotoxicity Evaluation of Jurkat Expressing Chimeric Antigen Receptor using Fluorescent Imaging
2.0K Views
•
09:20 min
•
October 27th, 2023
DOI :
October 27th, 2023
•Siddharth Subham*1,2,3, John D. Jeppson*1, Benjamin K. Gibbs4, Jacqueline Babai2, Riza Alker1, Andrew K. Godwin4,5,6, David Akhavan1,2,3
1Department of Radiation Oncology, University of Kansas Cancer Center, 2Department of Cancer Biology, University of Kansas Cancer Center, 3BioEngineering Program, University of Kansas, 4Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, 5University of Kansas Cancer Center, 6Kansas Institute for Precision Medicine, University of Kansas Medical Center
Chapters in this video
0:00
Introduction
1:42
Plating CFSE‐Labelled Tumor Cells and Co‐Culturing CAR‐Expressing Jurkats for In Vitro Cytotoxicity Evaluation
5:19
Preparation of Plate for Fluorescent Imaging and Cell Viability Assessment in CAR‐Jurkat Co‐Culture with CFSE‐Labeled Tumor Cells
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