Scoring Central Nervous System Inflammation, Demyelination, and Axon Injury in Experimental Autoimmune Encephalomyelitis

3.8K Views

•

08:17 min

•

February 23rd, 2024

DOI :

10.3791/65738-v

February 23rd, 2024

•

Carmen C. Ucciferri¹, Annette Gower², Nuria Alvarez-Sanchez¹^,², Heather Whetstone³, Valeria Ramaglia¹, Jennifer L. Gommerman¹, Koroboshka Brand-Arzamendi², Raphael Schneider²^,⁴, Shannon E Dunn¹^,²^,⁵

¹Department of Immunology, University of Toronto, ²Keenan Research Centre for Biomedical Science of St. Michael’s Hospital, ³Sickkids Research Institute, The Hospital for Sick Children, ⁴BARLO MS Centre, St. Michael’s Hospital, ⁵Women’s College Research Institute, Women’s College Hospital

Summary

Explore More Videos

Central Nervous System

Autoimmune Encephalomyelitis

Experimental Autoimmune Encephalomyelitis

Multiple Sclerosis

MS Risk Factors

Brain Inflammation

Immune Infiltration

Neurofilament Light

Chapters in this video

0:00

Introduction

1:48

Harvesting and Fixing Mouse Brain and Spinal Cord Tissues for Histological Scoring of Experimental Autoimmune Encephalomyelitis

3:15

Paraffin Embedding and Histopathological Scoring of Demyelinating Lesions and Perivascular Cuffs in Mouse Brain and Spinal Cord Tissues

Related Videos

article

Induction and Clinical Scoring of Chronic-Relapsing Experimental Autoimmune Encephalomyelitis

17.9K Views

article

Myelin Oligodendrocyte Glycoprotein (MOG_35-55) Induced Experimental Autoimmune Encephalomyelitis (EAE) in C57BL/6 Mice

81.5K Views

article

Induction of Experimental Autoimmune Encephalomyelitis in Mice and Evaluation of the Disease-dependent Distribution of Immune Cells in Various Tissues

23.5K Views

article

Visualizing Impairment of the Endothelial and Glial Barriers of the Neurovascular Unit during Experimental Autoimmune Encephalomyelitis In Vivo

7.6K Views

article

Flow Cytometric Analysis of Lymphocyte Infiltration in Central Nervous System during Experimental Autoimmune Encephalomyelitis

7.0K Views

article

Quantification of Autoreactive Antibodies in Mice upon Experimental Autoimmune Encephalomyelitis

729 Views

article

Simultaneous Isolation of Principal Central Nervous System-Resident Cell Types from Adult Autoimmune Encephalomyelitis Mice

1.6K Views

article

Role of CD4 T Cell in Relapsing-Remitting Experimental Autoimmune Encephalomyelitis

244 Views

article

Induction and Diverse Assessment Indicators of Experimental Autoimmune Encephalomyelitis

3.4K Views

article

Generation of Experimental Autoimmune Encephalomyelitis in a Mouse Model

84 Views

Copyright © 2025 MyJoVE Corporation. All rights reserved