Scoring Central Nervous System Inflammation, Demyelination, and Axon Injury in Experimental Autoimmune Encephalomyelitis

3.8K Views

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08:17 min

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February 23rd, 2024

DOI :

10.3791/65738-v

February 23rd, 2024

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Carmen C. Ucciferri¹, Annette Gower², Nuria Alvarez-Sanchez¹^,², Heather Whetstone³, Valeria Ramaglia¹, Jennifer L. Gommerman¹, Koroboshka Brand-Arzamendi², Raphael Schneider²^,⁴, Shannon E Dunn¹^,²^,⁵

¹Department of Immunology, University of Toronto, ²Keenan Research Centre for Biomedical Science of St. Michael’s Hospital, ³Sickkids Research Institute, The Hospital for Sick Children, ⁴BARLO MS Centre, St. Michael’s Hospital, ⁵Women’s College Research Institute, Women’s College Hospital

Summary

Explore More Videos

Central Nervous System

Autoimmune Encephalomyelitis

Experimental Autoimmune Encephalomyelitis

Multiple Sclerosis

MS Risk Factors

Brain Inflammation

Immune Infiltration

Neurofilament Light

Chapters in this video

0:00

Introduction

1:48

Harvesting and Fixing Mouse Brain and Spinal Cord Tissues for Histological Scoring of Experimental Autoimmune Encephalomyelitis

3:15

Paraffin Embedding and Histopathological Scoring of Demyelinating Lesions and Perivascular Cuffs in Mouse Brain and Spinal Cord Tissues

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