Surgical Perfusion and Isolation of the Porcine Pancreas for Islet Isolation

933 Views

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08:32 min

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June 7th, 2024

DOI :

10.3791/66350-v

June 7th, 2024

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Sophia H. Roberts¹^,², Louai Alrata¹^,², Dahlia Abdulsattar¹^,², Karan Joseph¹^,², Wahid Abu-Amer¹^,², Mohamed Zaghloul¹^,², Batool Arif¹^,², Tarek Alhamad³, Maria S. Remedi⁴, Yiing Lin⁵, Mohamed A. Zayed¹^,²^,⁶^,⁷

¹Department of Surgery, Section of Vascular Surgery, Washington University School of Medicine, ²CardioVascular Research Innovation in Surgery and Engineering Center, Washington University School of Medicine, ³Department of Medicine, Division of Nephrology, Washington University School of Medicine, ⁴Department of Medicine, Division of Endocrinology, Washington University School of Medicine, ⁵Department of Surgery, Section of Transplant Surgery, Washington University School of Medicine, ⁶Division of Molecular Cell Biology, Washington University School of Medicine, ⁷Department of Biomedical Engineering, Washington University McKelvey School of Engineering

Summary

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Transplantation Research

Chapters in this video

0:00

Introduction

1:49

Dissection and Surgical Perfusion of Porcine Pancreas for Islet Isolation

6:12

Continuous Gradient Method for Porcine Islet Purification

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