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Subculture and Cryopreservation of Esophageal Adenocarcinoma Organoids: Pros and Cons for Single Cell Digestion

DOI :

10.3791/63281-v

July 6th, 2022

July 6th, 2022

3,475 Views

1Department of General, Visceral, Cancer and Transplantation Surgery, University Hospital Cologne, 2Institute of Pathology, University Hospital Cologne

This protocol describes the methods of subculture and cryopreservation of esophageal adenocarcinoma organoids with and without single cell digestion to enable researchers to choose appropriate strategies based on their experimental design.

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Subculture

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