An Automated Microscopic Scoring Method for the γ-H2AX Foci Assay in Human Peripheral Blood Lymphocytes
4.7K Views
•
08:23 min
•
December 25th, 2021
DOI :
December 25th, 2021
•Shankari Nair1, Samantha Cairncross2, Xanthene Miles1, Monique Engelbrecht3, Peter du Plessis1, Julie Bolcaen1, Randall Fisher1, Roya Ndimba1, Charnay Cunningham4, Wilner Martínez-López5, Christian Schunck6, Charlot Vandevoorde1
1Radiation Biophysics Division, Nuclear Medicine Department, iThemba LABS, 2Department of Biotechnology, University of the Western Cape, 3Department of Medical Biosciences, University of the Western Cape, 4Division of Medical Physiology, Department of Medicine and Health Sciences, Stellenbosch University, 5Biodosimetry Service, Instituto de Investigaciones Biológicas Clemente Estable and Academic Unit on Radiation Protection, Faculty of Medicine, University of the Republic, 6MetaSystems Hard & Software GmbH
Chapters in this video
0:00
Introduction
0:44
Preparation of Samples
1:26
Sample Irradiations
1:35
Slide Preparation
2:17
Fixation and Immunofluorescence γ-H2AX Staining
3:44
Automated Metafer Scanning and Scoring
7:29
Results
8:03
Conclusion
Related Videos
07:00
Intra-iliac Artery Injection for Efficient and Selective Modeling of Microscopic Bone Metastasis
16.2K Views
04:15
A Mouse Model of Fatigue Induced by Peripheral Irradiation
7.8K Views
06:00
Development of a Human Preclinical Model of Osteoclastogenesis from Peripheral Blood Monocytes Co-cultured with Breast Cancer Cell Lines
7.0K Views
08:52
Modeling Osteosarcoma Using Li-Fraumeni Syndrome Patient-derived Induced Pluripotent Stem Cells
8.8K Views
10:09
Methods for Evaluating the Role of c-Fos and Dusp1 in Oncogene Dependence
8.2K Views
08:17
A Human Peripheral Blood Mononuclear Cell (PBMC) Engrafted Humanized Xenograft Model for Translational Immuno-oncology (I-O) Research
14.1K Views
08:36
In Vitro Assay to Study Tumor-macrophage Interaction
7.4K Views
07:58
An Automated Differential Nuclear Staining Assay for Accurate Determination of Mitocan Cytotoxicity
6.7K Views
05:18
Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells
11.0K Views
08:25
Detection of Cell-Free DNA in Blood Plasma Samples of Cancer Patients
10.8K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved