In Vivo 2-Photon Imaging of Neurons Fluorescently Labeled by Single-Cell Electroporation
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Conclusion
Single-cell electroporation (SCE) is a specialized technique allowing delivery of DNA or other macromolecules into individual cells within intact tissue, including in vivo preparations. Here we detail the procedure for SCE of a fluorescent dye or plasmid DNA into neurons within the intact brain of the Xenopus laevis tadpole.