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Culture of myeloid dendritic cells from bone marrow precursors

22.4K Views

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06:39 min

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July 25th, 2008

DOI :

10.3791/769-v

July 25th, 2008

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1Medical Sciences Program, McMaster University, 2Centre for Gene Therapeutics, McMaster University, 3Department of Chemical Engineering, University of Waterloo

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Myeloid Dendritic Cells
Bone Marrow Precursors
Immune Mechanisms
Antigen Presenting Cells
Vaccine Vector
Tibias And Femurs
In Vitro Differentiation Of DCs
Functional Activity Of DCs
Cell Surface Markers
Cytokine Production
Maturation Of DCs
Flow Cytometric Comparisons
Co stimulatory Molecules
IL 12 Cytokine
CpG Stimulation
Mock Treatment
Vaccine Vector Manipulation
Antigen specific Immune Responses
In Vitro Pulsing
Recombinant Viral Vectors

Chapters in this video

0:05

Title

0:08

Introduction

1:04

Harvest and Processing Of Bone Marrow

3:41

Culture of Dendritic Cells

5:07

Sample DC Microscropy and Flow Control

6:12

Conclusions

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