Isolation of Adeno-Associated Viral Vectors Through a Single-Step and Semi-Automated Heparin Affinity Chromatography Protocol

2.5K Views

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09:12 min

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April 5th, 2024

DOI :

10.3791/66550-v

April 5th, 2024

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Miguel M. Lopes*¹^,²^,³, Sara M. Lopes*¹^,²^,³, Rafael Baganha¹^,²^,⁴, Carina Henriques¹^,²^,⁵, Ana C. Silva¹^,²^,³, Diana D. Lobo¹^,²^,³, Luísa Cortes¹^,²^,³^,⁶, Luís Pereira de Almeida*¹^,²^,⁴^,⁵, Rui Jorge Nobre*¹^,²^,³^,⁴

¹CNC-UC - Center for Neuroscience and Cell Biology, University of Coimbra, ²CIBB - Center for Innovative Biomedicine and Biotechnology, University of Coimbra, ³IIIUC - Institute for Interdisciplinary Research, University of Coimbra, ⁴ViraVector - Viral Vectors for Gene Transfer Core Facility, University of Coimbra, ⁵FFUC - Faculty of Pharmacy, University of Coimbra, ⁶MICC-CNC - Microscopy Imaging Center of Coimbra - CNC, University of Coimbra

Summary

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Adeno Associated Virus AAV

Viral Vectors

Heparin Affinity Chromatography

Purification Protocol

Mosaic RAAVs

Transient Transfection

Chromatography Techniques

Scalability

Cost effectiveness

In Vivo Gene Delivery

Preclinical Studies

Ultracentrifugation

Heparan Sulfate Proteoglycans

Chapters in this video

0:00

Introduction

1:54

Production of Recombinant AAV by Transient Transfection

4:29

FPLC Purification and Characterization of Recombinant AAV

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