نحن ممتنون للغاية للدكتور فرانك كيرشوف لتوفير GFAP-EGFP وسلالات الفئران CX3CR1-EGFP. وأيد العمل من المنح المقدمة من مركز الحراك الدولي من فنلندا، تيكيس، كلية الدراسات العليا الفنلندية العلوم العصبية (FGSN) وأكاديمية فنلندا.

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والكتاب ليس لديهم ما يكشف.

الصدمة الدماغ هو، لا يمكن التنبؤ بها الحدث المفاجئ. هنا، نحن تصف نموذج الحيوان الذي يستنسخ مجموعة من التغيرات المرضية التي لوحظت في المرضى من البشر بعد إصابات الدماغ مثل التنكس العصبي، والقضاء على التشعبات، وذمة الدماغ، ندبة الدبقية، نزيف في قشرة الدماغ إلى جانب نز?...

إصابات الدماغ الحادة مشكلة صحية عامة كبيرة مع ارتفاع معدل الإصابة في حوادث السيارات، والسقوط أو الاعتداءات، وارتفاع معدل انتشار الإعاقة المزمنة لاحقة. يبقى النهج العلاجي لعلاج إصابات الدماغ أعراض تماما، مما يحد من فعالية الرعاية قبل دخول المستشفى والجراحية والحرجة. وهذا يجعل الأثر الاجتماعي والاقتصادي للإصابات الدماغ الحادة بشكل خاص. لمجموعة متنوعة من الأسباب، فإن معظم التجارب السريرية لم تثبت تحسنا في الانتعاش بعد إصابات الدماغ باستخدام أساليب علاجية جديدة. النماذج الحيوانية ذات أهمية حاسمة لتطوير استراتيجيات علاجية جديدة نحو مرحلة حيث يمكن التنبؤ نجاعة الأدوية في المرضى الذين يعانون من إصابات في المخ. في الوقت الحاضر، العديد من النماذج الحيوانية راسخة من صدمات الرأس موجودة، بما في ذلك تأثير تسيطر القشرية 1، السائل إصابة قرع 2، تشوه القشرية ديناميكية 3، الوزن الإفلات4، وإصابة 5 الصورة. وقد استخدمت عدد من نماذج تجريبية لدراسة بعض الجوانب الشكلية والجزيئية والسلوكية للرئيس علم الأمراض المرتبطة الصدمة. ومع ذلك، لا يوجد نموذج حيوان واحد هو ناجحة تماما في التحقق من صحة استراتيجيات علاجية جديدة. تطوير نماذج حيوانية يمكن الاعتماد عليها، والتي تسيطر استنساخه من إصابات الدماغ هو ضروري لتقييم العمليات المرضية المعقدة. مزيج رواية من أحدث تقنيات التصوير المجهري وصحفيين الفلورسنت المشفرة وراثيا يوفر فرصة غير مسبوقة لتحقيق في جميع مراحل إصابات الدماغ، والتي تشمل الإصابة الأولية، وانتشار الإصابة الأولية والإصابة الثانوية، والتجدد. على وجه الخصوص، في الجسم الحي اثنين من الفوتون المجهري هو التكنولوجيا البصرية غير الخطية الفريدة التي تسمح التصور في الوقت الحقيقي من الهياكل الخلوية وحتى التحت خلوية في الطبقات القشرية العميقة من الدماغ القوارض. عدة أنواع من الخلايا وورشولا يمكن تصوير nelles في وقت واحد من خلال الجمع بين علامات الفلورسنت مختلفة. باستخدام هذه الأداة القوية، يمكننا تصور التغيرات المورفولوجية والوظيفية الحيوية في الدماغ يعيشون في ظل ظروف ما بعد الصدمة. وقد أظهرت مزايا في الجسم الحي اثنين من الفوتون المجهري في دراسة إصابات الدماغ مؤخرا كيروف والزملاء 6. باستخدام نموذج كدمة خفيفة التنسيق القشرية، أظهر الباحثون أن هذه الإصابة شجيري الحاد في القشرة pericontusional وبوابات من انخفاض في تدفق الدم المحلية. وعلاوة على ذلك، فإنها أظهرت أن القشرة للخطر عملية الأيض حول الموقع كدمة تلف مزيد من الاستقطاب الانتشار. هذا الضرر الثانوي يؤثر الدوائر متشابك، مما يجعل عواقب إصابات في الدماغ أكثر شدة. هنا، نقترح طريقة وخز التجسيمي مع إبرة حقنة، والتي يمكن أن تكون مجتمعة في وقت واحد مع تطبيق المخدرات الموضعية، كنموذج متقدم للدماغ المحليةإصابة وكأداة لدراسة العواقب المرضية في جسم المريض من الصدمة الحادة في الدماغ في الثدييات في الجسم الحي.

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			<td align="right" style="width:119px;">50264</td>
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		<tr height="35">
			<td height="35" style="height:35px;width:216px;">UMP3 microsyringe pump and Micro 4 microsyringe pump controller</td>
			<td style="width:71px;">WPI</td>
			<td style="width:119px;">UMP3-1</td>
			<td style="width:167px;">Microinjector and controller</td>
		</tr>
		<tr height="35">
			<td height="35" style="height:35px;width:216px;">Xylazine</td>
			<td style="width:71px;">Bayer Health Care</td>
			<td style="width:119px;">Rompun vet</td>
			<td style="width:167px;"></td>
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</table>

acute brain trauma in mice followed by longitudinal two-photon imaging

على الرغم من الصدمة الدماغ الحادة غالبا ما ينجم عن تلف الرأس في حوادث مختلفة ويؤثر على جزء كبير من السكان، لا يوجد علاج فعال لذلك حتى الآن. القيود المفروضة على نماذج حيوانية المستخدمة حاليا تعيق فهم آلية الأمراض. Multiphoton المجهري يسمح دراسة الخلايا والأنسجة داخل أدمغة الحيوانات سليمة طوليا في ظل الظروف الفسيولوجية والمرضية. هنا، نحن تصف نموذجين من إصابات الدماغ الحادة درس عن طريق التصوير ثنائي الفوتون من السلوك خلايا الدماغ في ظل ظروف ما بعد الصدمة. وأصيب المنطقة من المخ اختيارها بإبرة حادة لإنتاج الصدمة بعرض للرقابة وعمق في لحمة الدماغ. يستخدم أسلوب لدينا وخز التجسيمي مع إبرة حقنة، والتي يمكن دمجها مع التطبيق المتزامن المخدرات. نقترح أن هذه الطريقة يمكن أن تستخدم أداة متقدمة لدراسة الآليات الخلوية من العواقب المرضية في جسم المريض من صدمة حادة في الدماغ في الثدييات <em&gt; في الجسم الحي. في هذا الفيديو، ونحن الجمع بين إصابات الدماغ الحادة مع اثنين من الاستعدادات: الجمجمة الجمجمة النافذة ورقيق. نحن أيضا مناقشة مزايا وقيود كل من الاستعدادات لتصوير مراحل متعددة من تجديد الدماغ بعد الصدمة.

لدينا اثنين الأمثل الإجراءات العملية: 1) نافذة الجمجمة المزمن و2) ترقق الجمجمة، لتصوير الدماغ ما بعد الصدمة في الفئران المعدلة وراثيا. ويرد تخطيطيا من التحضيرات تجريبية في الشكل 1. يتم تطبيق وخز الصدمة بواسطة إبرة الصلب من 0.3 مم OD (30 G) إلى حفر (1A الشكل) ?...

Watch this Scientific Journal Video about Acute Brain Trauma in Mice Followed By Longitudinal Two-photon Imaging at JoVE.com

Acute Brain Trauma in Mice Followed By Longitudinal Two-photon Imaging

الصدمة الدماغ الحاد هو إصابة الشديدة التي لا يوجد لديه العلاج المناسب حتى الآن. Multiphoton المجهري يسمح دراسة طوليا عملية التنمية الصدمة الدماغ الحادة وسبر الاستراتيجيات العلاجية في القوارض. وأظهرت نموذجين من الصدمة الدماغ الحادة درس في الجسم الحي مع اثنين من الفوتون التصوير من الدماغ في هذا البروتوكول.

الصدمة الحادة الدماغ في الفئران تليها الطولية التصوير ثنائي الفوتون

Although acute brain trauma often results from head damage in different accidents and affects a substantial fraction of the population, there is no ...

acute-brain-trauma-in-mice-followed-by-longitudinal-two-photon-imaging

Research

JoVE Journal

Medicine

12.3K Views.  University of Helsinki.  الصدمة الدماغ الحاد هو إصابة الشديدة التي لا يوجد لديه العلاج المناسب حتى الآن. Multiphoton المجهري يسمح دراسة طوليا عملية التنمية الصدمة الدماغ الحادة وسبر الاستراتيجيات العلاجية في القوارض. وأظهرت نموذجين من الصدمة الدماغ الحادة درس في ال?...

Video: Acute Brain Trauma in Mice Followed By Longitudinal Two-photon Imaging

Pseudofracture: An Acute Peripheral Tissue Trauma Model

Following trauma there is an early hyper-reactive inflammatory response that can lead to multiple organ dysfunction and high mortality in trauma patients; this response is often accompanied by a delayed immunosuppression that adds the clinical complications of infection and can also increase mortality.1-9 Many studies have begun to assess these changes in the reactivity of the immune system following trauma.10-15 
Immunologic studies are greatly supported through the wide variety of transgenic and knockout mice available for in vivo modeling; these strains aid in detailed investigations to assess the molecular pathways involved in the immunologic responses.16-21
The challenge in experimental murine trauma modeling is long term investigation, as fracture fixation techniques in mice, can be complex and not easily reproducible.22-30
This pseudofracture model, an easily reproduced trauma model, overcomes these difficulties by immunologically mimicking an extremity fracture environment, while allowing freedom of movement in the animals and long term survival without the continual, prolonged use of anaesthesia. The intent is to recreate the features of long bone fracture; injured muscle and soft tissue are exposed to damaged bone and bone marrow without breaking the native bone.
The pseudofracture model consists of two parts: a bilateral muscle crush injury to the hindlimbs, followed by injection of a bone solution into these injured muscles. The bone solution is prepared by harvesting the long bones from both hindlimbs of an age- and weight-matched syngeneic donor. These bones are then crushed and resuspended in phosphate buffered saline to create the bone solution.
Bilateral femur fracture is a commonly used and well-established model of extremity trauma, and was the comparative model during the development of the pseudofracture model. Among the variety of available fracture models, we chose to use a closed method of fracture with soft tissue injury as our comparison to the pseudofracture, as we wanted a sterile yet proportionally severe peripheral tissue trauma model. 31
Hemorrhagic shock is a common finding in the setting of severe trauma, and the global hypoperfusion adds a very relevant element to a trauma model. 32-36 The pseudofracture model can be easily combined with a hemorrhagic shock model for a multiple trauma model of high severity. 37

Pseudofracture, a reproducible murine model of sterile musculoskeletal trauma, allows for evaluation of late term post-traumatic immune responses. This article describes the procedural execution of the model step by step, including the potential for experimental model combinations to permit study of multiple trauma.

كسر كاذب : والصدمات الحادة الأنسجة الطرفية النموذجي

Following trauma there is an early hyper-reactive inflammatory response that can lead to multiple organ dysfunction and high mortality in trauma patients; ...

The Use of Pharmacological-challenge fMRI in Pre-clinical Research: Application to the 5-HT System

Pharmacological MRI (phMRI) is a new and promising method to study the effects of substances on brain function that can ultimately be used to unravel underlying neurobiological mechanisms behind drug action and neurotransmitter-related disorders, such as depression and ADHD. Like most of the imaging methods (PET, SPECT, CT) it represents a progress in the investigation of brain disorders and the related function of neurotransmitter pathways in a non-invasive way with respect of the overall neuronal connectivity. Moreover it also provides the ideal tool for translation to clinical investigations. MRI, while still behind in molecular imaging strategies compared to PET and SPECT, has the great advantage to have a high spatial resolution and no need for the injection of a contrast-agent or radio-labeled molecules, thereby avoiding the repetitive exposure to ionizing radiations. Functional MRI (fMRI) is extensively used in research and clinical setting, where it is generally combined with a psycho-motor task. phMRI is an adaptation of fMRI enabling the investigation of a specific neurotransmitter system, such as serotonin (5-HT), under physiological or pathological conditions following activation via administration of a specific challenging drug.
The aim of the method described here is to assess brain 5-HT function in free-breathing animals. By challenging the 5-HT system while simultaneously acquiring functional MR images over time, the response of the brain to this challenge can be visualized. Several studies in animals have already demonstrated that drug-induced increases in extracellular levels of e.g. 5-HT (releasing agents, selective re-uptake blockers, etc) evoke region-specific changes in blood oxygenation level dependent (BOLD) MRI signals (signal due to a change of the oxygenated/deoxygenated hemoglobin levels occurring during brain activation through an increase of the blood supply to supply the oxygen and glucose to the demanding neurons) providing an index of neurotransmitter function. It has also been shown that these effects can be reversed by treatments that decrease 5-HT availability16,13,18,7. In adult rats, BOLD signal changes following acute SSRI administration have been described in several 5-HT related brain regions, i.e. cortical areas, hippocampus, hypothalamus and thalamus9,16,15. Stimulation of the 5-HT system and its response to this challenge can be thus used as a measure of its function in both animals and humans2,11.

The goal of this technique is to assess serotonin (5-HT) neurotransmitter function in the live and free-breathing animal with pharmacological magnetic resonance imaging (phMRI) and an intravenous challenge with a selective serotonin reuptake inhibitor (SSRI), fluoxetine.

استعمال الدوائي، التحدي الرنين المغناطيسي الوظيفي في مرحلة ما قبل السريرية للبحوث: بالتطبيق على النظام 5-HT

Pharmacological MRI (phMRI) is a new and promising method to study the effects of substances on brain function that can ultimately be used to unravel ...

An Orthotopic Glioblastoma Mouse Model Maintaining Brain Parenchymal Physical Constraints and Suitable for Intravital Two-photon Microscopy

Glioblastoma multiforme (GBM) is the most aggressive form of brain tumors with no curative treatments available to date.
Murine models of this pathology rely on the injection of a suspension of glioma cells into the brain parenchyma following incision of the dura-mater. Whereas the cells have to be injected superficially to be accessible to intravital two-photon microscopy, superficial injections fail to recapitulate the physiopathological conditions. Indeed, escaping through the injection tract most tumor cells reach the extra-dural space where they expand abnormally fast in absence of mechanical constraints from the parenchyma.
Our improvements consist not only in focally implanting a glioma spheroid rather than injecting a suspension of glioma cells in the superficial layers of the cerebral cortex but also in clogging the injection site by a cross-linked dextran gel hemi-bead that is glued to the surrounding parenchyma and sealed to dura-mater with cyanoacrylate. Altogether these measures enforce the physiological expansion and infiltration of the tumor cells inside the brain parenchyma. Craniotomy was finally closed with a glass window cemented to the skull to allow chronic imaging over weeks in absence of scar tissue development.
Taking advantage of fluorescent transgenic animals grafted with fluorescent tumor cells we have shown that the dynamics of interactions occurring between glioma cells, neurons (e.g. Thy1-CFP mice) and vasculature (highlighted by an intravenous injection of a fluorescent dye) can be visualized by intravital two-photon microscopy during the progression of the disease.
The possibility to image a tumor at microscopic resolution in a minimally compromised cerebral environment represents an improvement of current GBM animal models which should benefit the field of neuro-oncology and drug testing.

We have established a cortical orthotopic glioblastoma model in mice for intravital two-photon microscopy that recapitulates the biophysical constraints normally at play during the growth of the tumor. A chronic glass window replacing the skull above the tumor enables the follow-up of the tumor progression over time by two-photon microscopy.

ومثلي الأشكال ورم أرومي نموذج الفأر القيود الحفاظ على الدماغ متني البدنية ومناسبة لحيوي داخلي ثنائي الفوتون المجهري

Glioblastoma multiforme (GBM) is the most aggressive form of brain tumors with no curative treatments available to date.
Murine models of this pathology ...

Ultrasound Imaging-guided Intracardiac Injection to Develop a Mouse Model of Breast Cancer Brain Metastases Followed by Longitudinal MRI

Breast cancer brain metastasis, occurring in 30% of breast cancer patients at stage IV, is associated with high mortality. The median survival is only 6 months. It is critical to have suitable animal models to mimic the hemodynamic spread of the metastatic cells in the clinical scenario. Here, we are introducing the use of small animal ultrasound imaging to guide an accurate injection of brain tropical breast cancer cells into the left ventricle of athymic nude mice. Longitudinal MRI is used to assessing intracranial initiation and growth of brain metastases. Ultrasound-guided intracardiac injection ensures not only an accurate injection and hereby a higher successful rate but also significantly decreased mortality rate, as compared to our previous manual procedure. In vivo high resolution MRI allows the visualization of hyperintense multifocal lesions, as small as 310 &#181;m in diameter on T2-weighted images at 3 weeks post injection. Follow-up MRI reveals intracranial tumor growth and increased number of metastases that distribute throughout the whole brain.

A breast cancer brain metastasis mouse model is established with ultrasound imaging-guided intracardiac injection of MDA-MB231/Br-GFP cells. Development of multifocal intracranial metastases has been monitored longitudinally using high-resolution 9.4 T MRI.

التصوير بالموجات فوق الصوتية الموجهة داخل القلب حقن لتطوير نموذج الفأر من الثدي الانبثاث سرطان الدماغ تلاه الطولية التصوير بالرنين المغناطيسي

Breast cancer brain metastasis, occurring in 30% of breast cancer patients at stage IV, is associated with high mortality. The median survival is only 6 ...

Candida albicans Biofilm Development on Medically-relevant Foreign Bodies in a Mouse Subcutaneous Model Followed by Bioluminescence Imaging

Candida albicans biofilm development on biotic and/or abiotic surfaces represents a specific threat for hospitalized patients. So far, C. albicans biofilms have been studied predominantly in vitro but there is a crucial need for better understanding of this dynamic process under in vivo conditions. We developed an in vivo subcutaneous rat model to study C. albicans biofilm formation. In our model, multiple (up to 9) Candida-infected devices are implanted to the back part of the animal. This gives us a major advantage over the central venous catheter model system as it allows us to study several independent biofilms in one animal. Recently, we adapted this model to study C. albicans biofilm development in BALB/c mice. In this model, mature C. albicans biofilms develop within 48 hr and demonstrate the typical three-dimensional biofilm architecture. The quantification of fungal biofilm is traditionally analyzed post mortem and requires host sacrifice. Because this requires the use of many animals to perform kinetic studies, we applied non-invasive bioluminescence imaging (BLI) to longitudinally follow up in vivo mature C. albicans biofilms developing in our subcutaneous model. C. albicans cells were engineered to express the Gaussia princeps luciferase gene (gLuc) attached to the cell wall. The bioluminescence signal is produced by the luciferase that converts the added substrate coelenterazine into light that can be measured. The BLI signal resembled cell counts obtained from explanted catheters. Non-invasive imaging for quantifying in vivo biofilm formation provides immediate applications for the screening and validation of antifungal drugs under in vivo conditions, as well as for studies based on host-pathogen interactions, hereby contributing to a better understanding of the pathogenesis of catheter-associated infections.

Candida albicans Biofilm Development on Medically-relevant Foreign Bodies in a Mouse Subcutaneous Model Followed by Bioluminescence Imaging

We present an experimental procedure of Candida albicans biofilm development in a mouse subcutaneous model. Fungal biofilms were quantified by determining the number of colony forming units and by a non-invasive bioluminescence imaging, where the amount of light that is produced corresponds with the number of viable cells.

المبيضات البيض بيوفيلم التنمية على الهيئات الأجنبية طبيا ذات الصلة في الماوس تحت الجلد نموذج تلاه ضوء بارد التصوير

Candida albicans biofilm development on biotic and/or abiotic surfaces represents a specific threat for hospitalized patients. So far, C. albicans ...

Longitudinal In Vivo Imaging of the Cerebrovasculature: Relevance to CNS Diseases

Remodeling of the brain vasculature is a common trait of brain pathologies. In vivo imaging techniques are fundamental to detect cerebrovascular plasticity or damage occurring overtime and in relation to neuronal activity or blood flow. In vivo two-photon microscopy allows the study of the structural and functional plasticity of large cellular units in the living brain. In particular, the thinned-skull window preparation allows the visualization of cortical regions of interest (ROI) without inducing significant brain inflammation. Repetitive imaging sessions of cortical ROI are feasible, providing the characterization of disease hallmarks over time during the progression of numerous CNS diseases. This technique accessing the pial structures within 250 &#956;m of the brain relies on the detection of fluorescent probes encoded by genetic cellular markers and/or vital dyes. The latter (e.g., fluorescent dextrans) are used to map the luminal compartment of cerebrovascular structures. Germane to the protocol described herein is the use of an in vivo marker of amyloid deposits, Methoxy-O4, to assess Alzheimer&#39;s disease (AD) progression. We also describe the post-acquisition image processing used to track vascular changes and amyloid depositions. While focusing presently on a model of AD, the described protocol is relevant to other CNS disorders where pathological cerebrovascular changes occur.

Longitudinal In Vivo Imaging of the Cerebrovasculature: Relevance to CNS Diseases

This manuscript describes a procedure to track the remodeling of the cerebrovasculature during amyloid plaque accumulation in vivo using longitudinal two-photon microscopy. A thinned-skull preparation enables the visualization of fluorescent dyes to assess the progression of cerebrovascular damage in a mouse model of Alzheimer&#39;s disease.

طولي<em&gt; في فيفو</em&gt; التصوير من Cerebrovasculature: الأقرب للأمراض الجهاز العصبي المركزي

Remodeling of the brain vasculature is a common trait of brain pathologies. In vivo imaging techniques are fundamental to detect cerebrovascular ...

A Minimally Invasive Model to Analyze Endochondral Fracture Healing in Mice Under Standardized Biomechanical Conditions

Bone healing models are necessary to analyze the complex mechanisms of fracture healing to improve clinical fracture treatment. During the last decade, an increased use of mouse models in orthopedic research was noted, most probably because mouse models offer a large number of genetically-modified strains and special antibodies for the analysis of molecular mechanisms of fracture healing. To control the biomechanical conditions, well-characterized osteosynthesis techniques are mandatory, also in mice. Here, we report on the design and use of a closed bone healing model to stabilize femur fractures in mice. The intramedullary screw, made of medical-grade stainless steel, provides through fracture compression an axial and rotational stability compared to the mostly used simple intramedullary pins, which show a complete lack of axial and rotational stability. The stability achieved by the intramedullary screw allows the analysis of endochondral healing. A large amount of callus tissue, received after stabilization with the screw, offers ideal conditions to harvest tissue for biochemical and molecular analyses. A further advantage of the use of the screw is the fact that the screw can be inserted into the femur with a minimally invasive technique without inducing damage to the soft tissue. In conclusion, the screw is a unique implant that can ideally be used in closed fracture healing models offering standardized biomechanical conditions.

This protocol describes a minimally invasive osteosynthesis technique using an intramedullary screw for standardized stabilization of femur fractures, which can be used to analyze endochondral bone healing in mice.

نموذج مينيملي لتحليل الكسر Endochondral الشفاء في الفئران تحت ظروف النشاط الحيوي موحدة

Bone healing models are necessary to analyze the complex mechanisms of fracture healing to improve clinical fracture treatment. During the last decade, an ...

Inducing Acute Lung Injury in Mice by Direct Intratracheal Lipopolysaccharide Instillation

Airway administration of lipopolysaccharide (LPS) is a common way to study pulmonary inflammation and acute lung injury (ALI) in small animal models. Various approaches have been described, such as the inhalation of aerosolized LPS as well as nasal or intratracheal instillation. The presented protocol describes a detailed step-by-step procedure to induce ALI in mice by direct intratracheal LPS instillation and perform FACS analysis of blood samples, bronchoalveolar lavage (BAL) fluid, and lung tissue. After intraperitoneal sedation, the trachea is exposed and LPS is administered via a 22 G venous catheter. A robust and reproducible inflammatory reaction with leukocyte invasion, upregulation of proinflammatory cytokines, and disruption of the alveolo-capillary barrier is induced within hours to days, depending on the LPS dosage used. Collection of blood samples, BAL fluid, and lung harvesting, as well as the processing for FACS analysis, are described in detail in the protocol. Although the use of the sterile LPS is not suitable to study pharmacologic interventions in infectious diseases, the described approach offers minimal invasiveness, simple handling, and good reproducibility to answer mechanistic immunological questions. Furthermore, dose titration as well as the use of alternative LPS preparations or mouse strains allow modulation of the clinical effects, which can exhibit different degrees of ALI severity or early vs. late onset of disease symptoms.

Presented here is a step-by-step procedure to induce acute lung injury in mice by direct intratracheal lipopolysaccharide instillation and to perform FACS analysis of blood samples, bronchoalveolar lavage fluid, and lung tissue. Minimal invasiveness, simple handling, good reproducibility, and titration of disease severity are advantages of this approach.

إحداث إصابات الرئة الحادة في الفئران عن طريق التبيل المباشر للكيس الشحمي داخل الرغامى

Airway administration of lipopolysaccharide (LPS) is a common way to study pulmonary inflammation and acute lung injury (ALI) in small animal models. ...

Orthopedic Robot-Assisted Femoral Neck System in the Treatment of Femoral Neck Fracture

Cannulated screw fixation is the main therapy for femoral neck fractures, especially in young patients. The traditional surgical procedure uses C-arm fluoroscopy to place the screw freehand and requires several guide wire adjustments, which increases the operation time and radiation exposure. Repeated drilling can also cause damage to the blood supply and bone quality of the femoral neck, which can be followed by complications such as screw loosening, nonunion, and femoral head necrosis. In order to make fixation more precise and reduce the incidence of complications, our team applied robot-assisted orthopedic surgery for screw placement using the femoral neck system to modify the traditional procedure. This protocol introduces how to import a patient's X-ray information into the system, how to perform screw path planning in software, and how the robotic arm assists in screw placement. Using this method, the surgeons can place the screw successfully the first time, improve the accuracy of the procedure, and avoid radiation exposure. The whole protocol includes the diagnosis of femoral neck fracture; the collection of intraoperative X-ray images; screw path planning in the software; precise placement of the screw under the assistance of the robotic arm by the surgeon; and verification of the implant placement.

This article introduces a method of robot-assisted orthopedic surgery for screw placement during the treatment of femoral neck fracture using the femoral neck system, which allows for more accurate screw placement, improved surgical efficiency, and fewer complications.

نظام عنق الفخذ بمساعدة الروبوت في علاج كسر عنق الفخذ

Cannulated screw fixation is the main therapy for femoral neck fractures, especially in young patients. The traditional surgical procedure uses C-arm ...

Urethral Stricture Induction Followed by Buccal Mucosa Graft Urethroplasty in a Rat Model

Urethral reconstruction is an important area of expertise for urologists. The buccal mucosa is considered the best option when urethral grafting is necessary, although in some cases, it is inappropriate or needs to be optimized to repair a given stricture. Therefore, developing innovative procedures and evaluating their putative success in experimental models is crucial to fit the clinical need. With this goal, this study describes a protocol in which urethral stricture was induced by electrocautery in Wistar rats. Urethral reconstruction was performed 1 week later with a buccal mucosa graft, harvested from the lower lip, and placed in a ventral onlay fashion. A retrograde urethrogram showed a significant improvement in urethral diameter after urethroplasty compared to the respective value after stricture induction. Additionally, the graft placement was assessed by blood perfusion analysis using laser Doppler. As expected, a dark blue area corresponded to the non-vascularized buccal mucosa graft. This procedure can successfully simulate the normal pathophysiological process of urethral injury and tissue modulation, as well as urethral reconstruction using a buccal mucosa graft in a reproducible manner, and serve as the basis for future research based on tissue engineering or urethral grafts.

In the present protocol, a urethral stricture induction was developed in Wistar rats, followed by urethral reconstruction with a buccal mucosa graft. A retrograde urethrogram and laser Doppler assessment were performed, validating urethral reconstruction (after stricture formation) and graft placement.

تحريض تضيق مجرى البول متبوعا برأب الإحليل بطعم الغشاء المخاطي الشدقي في نموذج الفئران

Urethral reconstruction is an important area of expertise for urologists. The buccal mucosa is considered the best option when urethral grafting is ...