JoVE Journal

Developmental Biology

This content is Open Access.

Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence

Transcript

The node and notochordal plate are transient signaling organizers in developing mouse embryos that can be visualized using several techniques. Here, we describe in detail how to perform two of the techniques to study their structure and morphogenesis: 1) scanning electron microscopy (SEM); and 2) whole mount immunofluorescence (WMIF).

Chapters in this video

0:04

Title

0:59

Scanning Electron Microscopy of the Mouse Embryonic Node

4:17

Whole Mount Immunofluorescence of the Mouse Node and Notochordal Plate

8:06

Results: Strip1 Mutant Embryos Display Irregular Nodes and Abnormal F-Actin Organization in the Axial Mesoderm

8:46

Conclusion

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