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Detecting SARS-CoV-2 Virus by Reverse Transcription-Loop-Mediated Isothermal Amplification

611 Views

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05:34 min

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September 8th, 2023

DOI :

10.3791/65662-v

September 8th, 2023

•

Steven A. David-Jimenez¹, Paola A. Caicedo¹, Maria F. Villegas-Torres¹^,², Natalia Campillo-Pedroza¹^,³

¹Facultad de Ingeniería, Diseño y Ciencias Aplicadas, Universidad Icesi, ²Centro de Investigaciones Microbiológicas (CIMIC), Department of Biological Sciences, Universidad de los Andes, ³BioDx: Diagnóstico y Soluciones Biotecnológicas S.A.S, Universidad Icesi

Summary

Here we provide a complete protocol to standardize and implement the method for detecting the SARS-CoV-2 virus in human samples by reverse transcription loop-mediated isothermal amplification (RT-LAMP). This method, done within 60 min, could be adapted to any laboratory or point-of-care at a low cost and using inexpensive equipment.

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Chapters in this video

0:00

Introduction

1:45

Reverse Transcriptase‐Loop Mediated Isothermal Amplification Reaction (RT‐LAMP) for SARS‐CoV-2 Detection

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