Concatemerization of Immuno-stimulatory DNA Sequences
2:43
DNA Purification and Analysis
5:55
Transfection of dsDNA Concatemers In Vitro
6:47
Transfection of dsDNA Concatemers In Vivo
8:29
Processing of DNA Stimulated Samples and Quantitative PCR Analysis
10:32
Results: Concatermerized DNA Stimulate an Innate Immune Response In Vitro and In Vivo
11:14
Conclusion
The aim of the protocol is to use optimal methods for stimulating the cytoplasmic DNA sensing pathways in cells and in vivo. This is achieved by improving the generation of long, double-stranded DNA during blunt-end ligation. Cells or mice are then transfected using a lipid transfection reagent.
