Extraction of Fibroblasts from Ear and Tail Tissues
3:20
Cell Culture and Sub-Culturing of Fibroblasts
5:05
Results: Fibroblast Culture Hardiness and Purity
6:03
Conclusion
副本
The overall goal of this protocol is to establish primary fibroblast cultures from the ears and tail of mice. This method can help answer key questions in the cell biology field, such as the difference between healthy and cancer cells. The main ad
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We describe a simple and quick experimental procedure for generating primary fibroblasts from the ears and tails of mice. The procedure does not require special animal training and can be used for the generation of fibroblast cultures from ears stored at RT for up to 10 days.
