Ultrasonic-augmented Primary Adult Fibroblast Isolation
July 29th, 2019
•We present a protocol to isolate primary adult fibroblasts in an easy, fast and reliable way, performable by beginners (e.g., students). The procedure combines enzymatic tissue digestion and mechanical agitation with ultrasonic waves to obtain primary fibroblasts. The protocol can easily be adapted to specific experimental requirements (e.g., human tissue).
Tags
Related Videos
Primary Culture of Human Vestibular Schwannomas
Primary Tumor and MEF Cell Isolation to Study Lung Metastasis
Isolation and Culture of Primary Endothelial Cells from Canine Arteries and Veins
Isolation and Cultivation of Adult Rat Cardiomyocytes
Isolation of Primary Human Decidual Cells from the Fetal Membranes of Term Placentae
Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method
Isolation of Atrial Myocytes from Adult Mice
Murine Myocardial Infarction Model using Permanent Ligation of Left Anterior Descending Coronary Artery
Refined CLARITY-Based Tissue Clearing for Three-Dimensional Fibroblast Organization in Healthy and Injured Mouse Hearts
Modifications of the Langendorff Method for Simultaneous Isolation of Atrial and Ventricular Myocytes from Adult Mice
