Results: Cue1 Is Required for Degradation of Deg1-Sec62
8:23
Conclusion
副本
The overall goal of this procedure is to visualize the degradation of the steady state population of a specific protein in Saccharomyces cerevisiae. This method can be used to determine the genetic requirements for, and environmental effects on, t
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Protein abundance reflects the rates of both protein synthesis and protein degradation. This article describes the use of cycloheximide chase followed by western blotting to analyze protein degradation in the model unicellular eukaryote, Saccharomyces cerevisiae (budding yeast).
