JoVE Logo

Sign In

Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae

28.8K Views

09:05 min

April 18th, 2016

DOI :

10.3791/53975-v

April 18th, 2016


Explore More Videos

Protein Degradation

Chapters in this video

0:05

Title

0:36

Growth and Harvest of Yeast Cells

3:14

Cycloheximide Chase

5:23

Post-alkaline Protein Extraction

6:51

Results: Cue1 Is Required for Degradation of Deg1-Sec62

8:23

Conclusion

Related Videos

article

10:09

Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes

1.9K Views

article

05:11

Tuning Degradation to Achieve Specific and Efficient Protein Depletion

6.1K Views

article

10:54

Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations

10.5K Views

article

07:47

Reporter-based Growth Assay for Systematic Analysis of Protein Degradation

10.6K Views

article

12:21

Measurement of mRNA Decay Rates in Saccharomyces cerevisiae Using rpb1-1 Strains

12.5K Views

article

10:57

Growth-based Determination and Biochemical Confirmation of Genetic Requirements for Protein Degradation in Saccharomyces cerevisiae

9.7K Views

article

08:40

Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae

1.3K Views

article

07:48

Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae

1.7K Views

article

10:48

An Introduction to Saccharomyces cerevisiae

231.9K Views

article

02:10

Misfolding-Prone Protein Degradation Assay: A Technique to Monitor Misfolded Protein Degradation Using Cycloheximide Treatment and Detergent Fractionation

197 Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved