May 18th, 2017
•This protocol describes a method to test the ability of a protein to co-sediment with filamentous actin (F-actin) and, if binding is observed, to measure the affinity of the interaction.
Related Videos
Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding
In Vitro Polymerization of F-actin on Early Endosomes
Measuring Biomolecular DSC Profiles with Thermolabile Ligands to Rapidly Characterize Folding and Binding Interactions
Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092
Measuring Trans-Plasma Membrane Electron Transport by C2C12 Myotubes
Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator
Measuring and Interpreting Oxygen Consumption Rates in Whole Fly Head Segments
High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase