Collecting Fertilized Eggs from Superovulated Mice
2:49
Preparation of Injection Pipette Containing the Lentiviral Vector, Micro-Injection
5:04
Transferring Embryos into B6CBAF1/JRj Pseudopregnant Females
8:10
Results: Various Tested Constructs
9:28
Conclusion
副本
This method allows rapid in vivo screening in mice, of the effect of a transgene, and is specifically adapted to perform gain or loss of function studies, as well an answer and DNA motive mapping. The main advantage of this technique, is that the
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Here, we present a protocol to promote transgene integration and production of founder transgenic mice with high efficacy by a simple injection of a lentiviral vector in the perivitelline space of a fertilized oocyte.