Plating Organoids and Co-culturing with Macrophages
5:27
Immunofluorescence Staining of Organoids
7:08
Results: Irradiated Epithelial Mammary Organoids were Cultured on Low-Adhesion Plates, Stained for Various Markers, and Co-Cultured with Immune Cells
8:51
Conclusion
副本
This protocol can be used and further developed to analyze how ionizing radiation affects tumor cell recruitment, leading to a greater understanding of cancer recurrence. Mammary organoids are powerful models. They mimic basic in vivo characterist
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Organoids developed from mouse mammary glands were irradiated and characterized to assess epithelial traits and interactions with immune cells. Irradiated organoids can be used to better evaluate cell-cell interactions that may lead to tumor cell recruitment in irradiated normal tissue.