Preparation of Agarose Molds and Generation of Endometrial Organoids
4:58
Harvesting Endometrial Organoids for Experiments
6:40
Results: Analysis of Generated Multicellular Human Primary Endometrial Organoids
8:10
Conclusion
副本
The endometrium is a tissue that changes every month in response to hormones. Hormone imbalance can prevent embryo attachment and also cause diseases like cancer. Our protocol rebuilds the endometrium so that it can be studied outside of the woman
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A protocol to generate human primary endometrial organoids that consist of epithelial and stromal cells and retain characteristics of the native endometrial tissue is presented. This protocol describes methods from uterine tissue acquisition to the histologic processing of endometrial organoids.
