Plating Organoids and Co-culturing with Macrophages
5:27
Immunofluorescence Staining of Organoids
7:08
Results: Irradiated Epithelial Mammary Organoids were Cultured on Low-Adhesion Plates, Stained for Various Markers, and Co-Cultured with Immune Cells
8:51
Conclusion
필기록
This protocol can be used and further developed to analyze how ionizing radiation affects tumor cell recruitment, leading to a greater understanding of cancer recurrence. Mammary organoids are powerful models. They mimic basic in vivo characterist
이 콘텐츠에 액세스하려면 로그인하거나 무료 평가판을 시작하세요
Organoids developed from mouse mammary glands were irradiated and characterized to assess epithelial traits and interactions with immune cells. Irradiated organoids can be used to better evaluate cell-cell interactions that may lead to tumor cell recruitment in irradiated normal tissue.