Results: Iron Treatment Increases ROS in Colorectal Cancer Cells
4:07
Conclusion
副本
This method can be used to analyze cellular reactive oxygen species in adherent cells with only a fluorescence microscope, and quantify reactive oxygen species intensity with fluorescence plate reader. This protocol is simple, efficient, and cost-
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Here, we present a protocol to detect total cellular reactive oxygen species (ROS) using 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA). This method can visualize cellular ROS localization in adherent cells with a fluorescence microscope and quantify ROS intensity with a fluorescence plate reader. This protocol is simple, efficient and cost-effective.