Our protocol can be used to improve the quality and quantity of gene expression data generated from FFPE-derived RNA samples, especially in suboptimal quality samples. This technique assesses the RNA quality within FFPE tissue samples and enables
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This method describes the steps to improve the quality and quantity of sequence data that can be obtained from formalin-fixed paraffin-embedded (FFPE) RNA samples. We describe the methodology to more accurately assess the quality of FFPE-RNA samples, prepare sequencing libraries, and analyze the data from FFPE-RNA samples.