Results: Patch-clamp and Synaptic Plasticity Measurements in Hippocampal Slices from Mice at P120 180
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Conclusion
副本
The protocol presented here diminishes excessive hypoxic damage during preparation of adult and aging mouse hippocampal slices, thus removing a major obstacle for studying function of mature and aging neural circuits. Introduction of hypothermia i
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This is a protocol for acute slice preparation from adult and aging mouse hippocampi that takes advantage of transcardial perfusion and slice cutting with ice-cold NMDG-aCSF to reduce hypoxic damage to the tissue. The resulting slices stay healthy over many hours, and are suitable for long-term patch-clamp and field-recordings.
