The Automated Detection and Analysis Of Exocytosis software will allow the user to automatically detect exocytic events and TIRF image sequences of pH-sensitive fluorophores. It will also automatically output features of exocytosis such as the spa
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We developed automated computer vision software to detect exocytic events marked by pH-sensitive fluorescent probes. Here, we demonstrate the use of a graphical user interface and RStudio to detect fusion events, analyze and display spatiotemporal parameters of fusion, and classify events into distinct fusion modes.