<meta charset="utf8"></head><body>蛋白质动力学研究中 NMR 15N 弛豫和 hetNOE 实验的方案

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			<td>Bruker 600 MHz AVANCE III HD spectrometer&#160;</td>
			<td>Bruker</td>
			<td>https://www.bruker.com/en/products-and-solutions/mr/nmr/avance-nmr-spectrometer.html</td>
			<td>NMR experiments conducted&#160;</td>
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nmr 15n relaxation experiments for the investigation of picosecond to nanoseconds structural dynamics of proteins

<meta charset="utf8"></head><body>作者聚焦：通过 NMR 弛豫实验探索内在无序的蛋白质动力学

NMR 15N 弛豫实验，用于研究蛋白质皮秒至纳秒的结构动力学

We investigate the structural dynamics of proteins by NMR spectroscopy to understand the molecular function better. NMR stands for Nuclear Magnetic Resonance Spectroscopy. It reduces effect that the nuclear spins of hydrogen atoms can transition between two energetically different states, and an auto magnetic field.We can use NMR spectroscopy to study the structural dynamics of intrinsically disordered proteins. These proteins are highly flexible, constantly changing shape, and frequently only fold into a stable structure when they interact with other molecules. We use NMR relaxation experiments to study how the protein backbone moves over very short time scales, ranging from picoseconds to nanoseconds, while focusing on each specific residue along the protein chain.This protocol will guide you step-by-step through setting up NMR relaxation experiments on an NMR spectrometer aimed at scientists with basic NMR knowledge, it ensures a fast, accurate setup, and introduces key concepts in protein backbone dynamics in an accessible way. This protocol makes NMR relaxation pulse sequences easily accessible to scientists studying protein dynamics. NMR relaxation experiments provide residue specific dynamic insights.Using this experimental approach, you can better understand backbone dynamics in proteins, the impact of protein-protein interaction on structured dynamics, and structural changes in the response to environmental changes.

Nuclear magnetic resonance (NMR) spectroscopy allows studying proteins in solution and under physiological temperatures. Frequently, either the amide ...

nmr-15n-relaxation-experiments-for-investigation-picosecond-to

Research

JoVE Journal

Biochemistry

NMR 15N Relaxation Experiments for the Investigation of Picosecond to Nanoseconds Structural Dynamics of Proteins

517 次观看.  Forschungszentrum Jülich. 我们通过 NMR 波谱研究蛋白质的结构动力学，以更好地了解分子功能。NMR 代表核磁共振波谱。它降低了氢原子的核自旋可以在两种能量不同的状态和自磁场之间转变的影响。我们可以使用 NMR 波谱来研究固有无序蛋白质的结构动力学。这些蛋白质具有高度柔韧性，形状不断变化，并且通常只有在与其他分子相互作用时才会折叠成稳定的结构。我?...