<meta charset="utf8"></head><body>단백질 역학 연구에서 NMR 15N 이완 및 hetNOE 실험을 위한 프로토콜

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			<td>Bruker 600 MHz AVANCE III HD spectrometer&#160;</td>
			<td>Bruker</td>
			<td>https://www.bruker.com/en/products-and-solutions/mr/nmr/avance-nmr-spectrometer.html</td>
			<td>NMR experiments conducted&#160;</td>
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nmr 15n relaxation experiments for the investigation of picosecond to nanoseconds structural dynamics of proteins

<meta charset="utf8"></head><body>저자 스포트라이트: NMR 이완 실험을 통한 본질적으로 정렬되지 않은 단백질 역학 탐색

단백질의 피코초에서 나노초까지의 구조 역학 조사를 위한 NMR 15N 이완 실험

We investigate the structural dynamics of proteins by NMR spectroscopy to understand the molecular function better. NMR stands for Nuclear Magnetic Resonance Spectroscopy. It reduces effect that the nuclear spins of hydrogen atoms can transition between two energetically different states, and an auto magnetic field.We can use NMR spectroscopy to study the structural dynamics of intrinsically disordered proteins. These proteins are highly flexible, constantly changing shape, and frequently only fold into a stable structure when they interact with other molecules. We use NMR relaxation experiments to study how the protein backbone moves over very short time scales, ranging from picoseconds to nanoseconds, while focusing on each specific residue along the protein chain.This protocol will guide you step-by-step through setting up NMR relaxation experiments on an NMR spectrometer aimed at scientists with basic NMR knowledge, it ensures a fast, accurate setup, and introduces key concepts in protein backbone dynamics in an accessible way. This protocol makes NMR relaxation pulse sequences easily accessible to scientists studying protein dynamics. NMR relaxation experiments provide residue specific dynamic insights.Using this experimental approach, you can better understand backbone dynamics in proteins, the impact of protein-protein interaction on structured dynamics, and structural changes in the response to environmental changes.

Nuclear magnetic resonance (NMR) spectroscopy allows studying proteins in solution and under physiological temperatures. Frequently, either the amide ...

nmr-15n-relaxation-experiments-for-investigation-picosecond-to

Research

JoVE Journal

Biochemistry

NMR 15N Relaxation Experiments for the Investigation of Picosecond to Nanoseconds Structural Dynamics of Proteins

574 조회수.  Forschungszentrum Jülich. 우리는 분자 기능을 더 잘 이해하기 위해 NMR 분광법으로 단백질의 구조 역학을 조사합니다. NMR은 Nuclear Magnetic Resonance Spectroscopy의 약자입니다. 그것은 수소 원자의 핵 스핀이 에너지적으로 다른 두 상태와 자동 자기장 사이에서 전이될 수 있는 효과를 줄입니다.NMR 분광법을 사용하여 본질적으로 무질서한 단백질의 구조 역학을 연구할 수 있습니...