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11:37 min
July 8th, 2010
DOI :
10.3791/2050-v
Kapitel
0:06
Title
0:48
Introduction
1:15
Reagent Preparation
1:56
Cell Preparation
3:11
Calpain Assay in Fixed Cells
4:48
Data Acquisition (Fixed Cells)
6:55
Calpain Assay in Live Cells
8:22
Calpain Assay in Mixed Cells
10:05
Analysis and Representative Results
11:09
Conclusion
Summary
This article will detail the protocol for measuring calpain activity in fixed and living cells using flow cytometry.
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