Simultane Multicolor-Imaging biologischer Strukturen mit Fluoreszenzphotoaktivierung Lokalisationsmikroskopie

We demonstrate the use of fluorescence photo activation localization microscopy (FPALM) to simultaneously image multiple types of fluorescently labeled molecules within cells. The techniques described yield the localization of thousands to hundreds of thousands of individual fluorescent labeled proteins, with a precision of tens of nanometers within single cells.