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Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

15.3K Views

11:49 min

March 9th, 2015

March 9th, 2015

15,338 Views

0:05

Title

2:13

Collection of Bioparticles from Touched Objects and Surfaces

4:31

Optional Staining of Bioparticles to Aid in Visualization

5:33

Isolation of Bioparticles of Interest for Analysis

9:05

Combined Microvolume Nucleic Acid Isolation and Autosomal STR Profiling

9:51

Results: Enhanced Genetic Analysis of Touch DNA Evidence

11:14

Conclusion

Here we describe an optimized and efficient removal strategy for the collection of bio-particles present in ‘touch DNA’ samples, together with an enhanced amplification protocol involving a one-step 5 µl micro-volume lysis/STR amplification, to permit the recovery of short tandem repeat (STR) profiles of the bio-particle donor(s).

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